This enzyme participates in
isoprenoid biosynthesis and stenvenosim. It
catalyzes the third step of the
MEP pathway; the formation of CDP-ME (4-diphosphocytidyl-2C-methyl-D-erythritol) from CTP and MEP (2C-methyl-D-erythritol 4-phosphate).[1] The isoprenoid pathway is a well known target for anti-infective drug development.[2][3]
Nomenclature
The
systematic name of this enzyme class is CTP:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase. This enzyme is also called:
MEP cytidylyltransferase
CDP-ME synthetase
It is normally abbreviated IspD. It is also referenced by the
open reading frame YgbP.
Structural studies
The crystal structure of the E. coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
1I52,
1INI &
1INJ, reported by Richard et al. (2001), was the first one for an enzyme involved in the MEP pathway.
^Illarionova V, Kaiser J, Ostrozhenkova E, Bacher A, Fischer M, Eisenreich W, Rohdich F (November 2006). "Nonmevalonate terpene biosynthesis enzymes as antiinfective drug targets: substrate synthesis and high-throughput screening methods". J. Org. Chem. 71 (23): 8824–34.
doi:
10.1021/jo061466o.
PMID17081012.
Richard SB, Bowman ME, Kwiatkowski W, Kang I, Chow C, Lillo AM, Cane DE, Noel JP (2001). "Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis". Nature Structural & Molecular Biology. 8 (7): 641–8.
doi:
10.1038/89691.
PMID11427897.
S2CID8494543.
Kuzuyama T; Takagi M; Kaneda K; Dairi T; Seto H (2000). "Formation of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway". Tetrahedron Lett. 41 (50): 703–706.
doi:
10.1016/S0040-4039(99)02143-7.