From Wikipedia, the free encyclopedia
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (
EC
2.3.1.191 , UDP-3-O-acyl-glucosamine N-acyltransferase , UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase , acyltransferase LpxD , acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase , firA (gene) , lpxD (gene) ) is an
enzyme with
systematic name (3R)-3-hydroxymyristoyl-(acyl-carrier protein):UDP-3-O-((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine N-acetyltransferase .
[1]
[2]
[3]
[4]
[5] This enzyme
catalyses the following
chemical reaction
(3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine
⇌
{\displaystyle \rightleftharpoons }
UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + holo-[acyl-carrier protein]
The enzyme catalyses a step of
lipid A biosynthesis.
References
^ Bartling CM, Raetz CR (September 2009).
"Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis" . Biochemistry . 48 (36): 8672–83.
doi :
10.1021/bi901025v .
PMC
2748855 .
PMID
19655786 .
^ Buetow L, Smith TK, Dawson A, Fyffe S, Hunter WN (March 2007).
"Structure and reactivity of LpxD, the N-acyltransferase of lipid A biosynthesis" . Proceedings of the National Academy of Sciences of the United States of America . 104 (11): 4321–6.
Bibcode :
2007PNAS..104.4321B .
doi :
10.1073/pnas.0606356104 .
PMC
1810333 .
PMID
17360522 .
^ Bartling CM, Raetz CR (May 2008).
"Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis" . Biochemistry . 47 (19): 5290–302.
doi :
10.1021/bi800240r .
PMC
2435086 .
PMID
18422345 .
^ Kelly TM, Stachula SA, Raetz CR, Anderson MS (September 1993).
"The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis" . The Journal of Biological Chemistry . 268 (26): 19866–74.
doi :
10.1016/S0021-9258(19)36593-7 .
PMID
8366125 .
^ Bainbridge BW, Karimi-Naser L, Reife R, Blethen F, Ernst RK, Darveau RP (July 2008).
"Acyl chain specificity of the acyltransferases LpxA and LpxD and substrate availability contribute to lipid A fatty acid heterogeneity in Porphyromonas gingivalis" . Journal of Bacteriology . 190 (13): 4549–58.
doi :
10.1128/jb.00234-08 .
PMC
2446808 .
PMID
18456814 .
External links
Activity Regulation Classification Kinetics Types