Carnitine palmitoyltransferase I (CPT1) also known as carnitine acyltransferase I, CPTI, CAT1, CoA:carnitine acyl transferase (CCAT), or palmitoylCoA transferase I, is a
mitochondrialenzyme responsible for the formation of acyl carnitines by catalyzing the transfer of the acyl group of a long-chain fatty acyl-CoA from coenzyme A to
l-carnitine. The product is often
Palmitoylcarnitine (thus the name), but other fatty acids may also be substrates.[5][6] It is part of a family of enzymes called carnitine acyltransferases.[7] This "preparation" allows for subsequent movement of the acyl carnitine from the
cytosol into the intermembrane space of mitochondria.
Three
isoforms of CPT1 are currently known: CPT1A, CPT1B, and CPT1C. CPT1 is associated with the
outer mitochondrial membrane. This enzyme can be inhibited by
malonyl CoA, the first committed intermediate produced during fatty acid synthesis. Its role in
fatty acid metabolism makes CPT1 important in many metabolic disorders such as
diabetes. Since its
crystal structure is not known, its exact mechanism of action remains to be determined.
Structure
CPT1 is an
integral membrane protein that exists in three isoforms in mammalian tissues: CPT1A, CPT1B and CPT1C. The first two are expressed on the outer mitochondrial membrane of most tissues, but their relative proportions varies between tissues. CPT1A predominates in lipogenic tissues like liver, whereas CPT1B predominates in tissues like heart and skeletal muscle that have a high fatty acid oxidative capacity
brown adipose cells.[8][9] Both isoforms are integral proteins of the mitochondrial outer membrane through two transmembrane regions in the
peptide chain. The membrane topology of CPT1A was described by [10] It is polytopic, with both the N- and C-termini exposed on the cytosolic aspect of the OMM, with a short loop linking the two transmembrane domains protruding into the mitochondrial inter-membrane space.
The third isoform (CPT1C), was identified in 2002 and is expressed in both mitochondria and the endoplasmic reticulum.[11] It is normally expressed only in neurones (brain), although its expression is altered in certain cancer cell types.[12][13]
The exact structure of any of the CPT1 isoforms has not yet been determined, although a variety of in silico models for CPT1 have been created based on closely related carnitine acyltransferases, such as
carnitine acetyltransferase (CRAT).[14]
An important structural difference between CPT1 and CPT2, CRAT and
carnitine octanoyltransferase (COT) is that CPT1 contains an additional domain at its
N-terminal consisting of about 160 amino acids. It has been determined that this additional N-terminal domain is important for the key inhibitory molecule of CPT1, malonyl-CoA, and acts like a switch that makes CPT1A more or less sensitive to malonyl-CoA inhibition [15]
Two distinct
binding sites have been proposed to exist in CPT1A and CPT1B. The "A site" or "CoA site" appears to bind both malonyl-CoA and
palmitoyl-CoA, as well as other molecules containing
coenzyme A, suggesting that the enzyme binds these molecules via interaction with the coenzyme A moiety. It has been suggested that malonyl-CoA may behave as a
competitive inhibitor of CPT1A at this site. A second "O site" has been proposed to bind malonyl-CoA more tightly than the A site. Unlike the A site, the O site binds to malonyl-CoA via the dicarbonyl group of the
malonate moiety of malonyl-CoA. The binding of malonyl-CoA to either the A and O sites inhibits the action of CPT1A by excluding the binding of carnitine to CPT1A.[16] Since a crystal structure of CPT1A has yet to be isolated and imaged, its exact structure remains to be elucidated.
Function
Enzyme mechanism
Because crystal structure data is currently unavailable, the exact mechanism of CPT1 is not currently known. A couple different possible mechanisms for CPT1 have been postulated, both of which include the
histidineresidue 473 as the key catalytic residue. One such mechanism based upon a carnitine acetyltransferase model is shown below in which the His 473 deprotonates carnitine while a nearby
serine residue stabilizes the tetrahedral
oxyanion intermediate.[7]
A different mechanism has been proposed that suggests that a
catalytic triad composed of residues Cys-305, His-473, and Asp-454 carries out the acyl-transferring step of
catalysis.[17] This catalytic mechanism involves the formation of a thioacyl-enzyme
covalent intermediate with Cys-305.
Biological function
The carnitine palmitoyltransferase system is an essential step in the
beta-oxidation of
long chain fatty acids. This transfer system is necessary because, while fatty acids are activated (in the form of a
thioester linkage to coenzyme A) on the outer mitochondrial membrane, the activated fatty acids must be oxidized within the
mitochondrial matrix. Long chain fatty acids such as palmitoyl-CoA, unlike short- and medium-chain fatty acids, cannot freely
diffuse through the
mitochondrial inner membrane, and require a shuttle system to be transported to the mitochondrial matrix.[18]
Carnitine palmitoyltransferase I is the first component and
rate-limiting step of the carnitine palmitoyltransferase system, catalyzing the transfer of the acyl group from coenzyme A to carnitine to form
palmitoylcarnitine. A
translocase then shuttles the acyl carnitine across the inner mitochondrial membrane where it is converted back into palmitoyl-CoA.
By acting as an acyl group acceptor, carnitine may also play the role of regulating the intracellular CoA:acyl-CoA ratio.[19]
Regulation
CPT1 is inhibited by malonyl-CoA, although the exact mechanism of inhibition remains unknown. The CPT1 skeletal muscle and heart isoform, CPT1B, has been shown to be 30-100-fold more sensitive to malonyl-CoA inhibition than CPT1A. This inhibition is a good target for future attempts to regulate CPT1 for the treatment of metabolic disorders.[20]
Acetyl-CoA carboxylase (ACC), the enzyme that catalyzes the formation of malonyl-CoA from
acetyl-CoA, is important in the regulation of fatty acid metabolism. Scientists have demonstrated that ACC2
knockout mice have reduced body fat and weight when compared to
wild type mice. This is a result of decreased activity of ACC which causes a subsequent decrease in malonyl-CoA concentrations. These decreased malonyl-CoA levels in turn prevent inhibition of CPT1, causing an ultimate increase in fatty acid oxidation.[21] Since heart and skeletal muscle cells have a low capacity for fatty acid synthesis, ACC may act purely as a regulatory enzyme in these cells.
CPT1 is associated with
type 2 diabetes and
insulin resistance. Such diseases, along with many other health problems, cause free fatty acid (FFA) levels in humans to become elevated, fat to accumulate in skeletal muscle, and decreases the ability of muscles to oxidize fatty acids. CPT1 has been implicated in contributing to these symptoms. The increased levels of malonyl-CoA caused by
hyperglycemia and
hyperinsulinemia inhibit CPT1, which causes a subsequent decrease in the transport of long chain fatty acids into muscle and heart mitochondria, decreasing fatty acid oxidation in such cells. The shunting of LCFAs away from mitochondria leads to the observed increase in FFA levels and the accumulation of fat in skeletal muscle.[24][25]
Its importance in fatty acid metabolism makes CPT1 a potentially useful enzyme to focus on in the development of treatments of many other metabolic disorders as well.[26]
Interactions
CPT1 is known to interact with many proteins, including ones from the NDUF family, PKC1, and ENO1.[27]
In HIV, Vpr enhances PPARbeta/delta-induced PDK4, carnitine palmitoyltransferase I (CPT1) mRNA expression in cells.[28] Knockdown of CPT1A by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells.[29]
^"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^van der Leij FR, Huijkman NC, Boomsma C, Kuipers JR, Bartelds B (2000). "Genomics of the human carnitine acyltransferase genes". Molecular Genetics and Metabolism. 71 (1–2): 139–53.
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10.1006/mgme.2000.3055.
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^Bonnefont JP, Djouadi F, Prip-Buus C, Gobin S, Munnich A, Bastin J (2004). "Carnitine palmitoyltransferases 1 and 2: biochemical, molecular and medical aspects". Molecular Aspects of Medicine. 25 (5–6): 495–520.
doi:
10.1016/j.mam.2004.06.004.
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^Price N, van der Leij F, Jackson V, Corstorphine C, Thomson R, Sorensen A, Zammit V (Oct 2002). "A novel brain-expressed protein related to carnitine palmitoyltransferase I". Genomics. 80 (4): 433–442.
doi:
10.1006/geno.2002.6845.
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^Shi J, Zhu H, Arvidson DN, Woldegiorgis G (Feb 2000). "The first 28 N-terminal amino acid residues of human heart muscle carnitine palmitoyltransferase I are essential for malonyl CoA sensitivity and high-affinity binding". Biochemistry. 39 (4): 712–717.
doi:
10.1021/bi9918700.
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