From Wikipedia, the free encyclopedia
Protein-coding gene in the species Homo sapiens
RPA1
Available structures
PDB Ortholog search:
PDBe
RCSB List of PDB id codes
1EWI ,
1FGU ,
1JMC ,
1L1O ,
2B29 ,
2B3G ,
4IJH ,
4IJL ,
4IPC ,
4IPD ,
4IPG ,
4IPH ,
4LUO ,
4LUV ,
4LUZ ,
4LW1 ,
4LWC ,
4NB3 ,
4O0A ,
4R4C ,
4R4I ,
4R4O ,
4R4Q ,
4R4T ,
5E7N ,
5EAY
Identifiers
Aliases
RPA1 , HSSB, MST075, REPA1, RF-A, RP-A, RPA70, Replication protein A1, PFBMFT6External IDs
OMIM :
179835 ;
MGI :
1915525 ;
HomoloGene :
2208 ;
GeneCards :
RPA1 ;
OMA :
RPA1 - orthologs
RNA expression pattern
Bgee
Human
Mouse (ortholog)
Top expressed in secondary oocyte ventricular zone ganglionic eminence parietal pleura visceral pleura germinal epithelium gingival epithelium trabecular bone tibia parotid gland
Top expressed in fetal liver hematopoietic progenitor cell retinal pigment epithelium ciliary body primitive streak vestibular membrane of cochlear duct iris Epithelium of choroid plexus abdominal wall endothelial cell of lymphatic vessel Paneth cell
More reference expression data
BioGPS
Wikidata
Replication protein A 70 kDa DNA-binding subunit is a
protein that in humans is encoded by the RPA1
gene .
[5]
Interactions
Replication protein A1 has been shown to
interact with:
BRCA2 ,
[6]
BLM ,
[7]
[8]
[9]
MCM2 ,
[10]
MCM4 ,
[10]
MCM6 ,
[10]
MCM7 ,
[10]
MUTYH ,
[11]
ORC2L ,
[10]
ORC6L ,
[10]
P53 ,
[12]
[13]
RPA2 ,
[14]
[15]
RPA3 ,
[15]
[16]
TIPIN ,
[17]
TP53BP1 ,
[18] and
XPA .
[19]
See also
References
^
a
b
c
GRCh38: Ensembl release 89: ENSG00000132383 –
Ensembl , May 2017
^
a
b
c
GRCm38: Ensembl release 89: ENSMUSG00000000751 –
Ensembl , May 2017
^
"Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Umbricht CB, Erdile LF, Jabs EW,
Kelly TJ (April 1993).
"Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A" . J Biol Chem . 268 (9): 6131–8.
doi :
10.1016/S0021-9258(18)53229-4 .
PMID
8454588 .
^ Wong JM, Ionescu D, Ingles CJ (January 2003).
"Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2" . Oncogene . 22 (1): 28–33.
doi :
10.1038/sj.onc.1206071 .
PMID
12527904 .
^ Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (August 2000).
"Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity" . J. Biol. Chem . 275 (31): 23500–8.
doi :
10.1074/jbc.M001557200 .
hdl :
10026.1/10318 .
PMID
10825162 .
^ Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (October 2002).
"Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases" . J. Biol. Chem . 277 (43): 41110–9.
doi :
10.1074/jbc.M205396200 .
PMID
12181313 .
^ Moens PB, Kolas NK, Tarsounas M, Marcon E, Cohen PE, Spyropoulos B (April 2002). "The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination". J. Cell Sci . 115 (Pt 8): 1611–22.
doi :
10.1242/jcs.115.8.1611 .
PMID
11950880 .
^
a
b
c
d
e
f Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol . 327 (1): 111–28.
doi :
10.1016/s0022-2836(03)00079-2 .
PMID
12614612 .
^ Parker A, Gu Y, Mahoney W, Lee SH, Singh KK, Lu AL (February 2001).
"Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair" . J. Biol. Chem . 276 (8): 5547–55.
doi :
10.1074/jbc.M008463200 .
PMID
11092888 .
^ Romanova LY, Willers H, Blagosklonny MV, Powell SN (Dec 2004). "The interaction of p53 with replication protein A mediates suppression of homologous recombination". Oncogene . 23 (56): 9025–33.
doi :
10.1038/sj.onc.1207982 .
PMID
15489903 .
S2CID
23482723 .
^ Riva F, Zuco V, Vink AA, Supino R, Prosperi E (Dec 2001). "UV-induced DNA incision and proliferating cell nuclear antigen recruitment to repair sites occur independently of p53-replication protein A interaction in p53 wild type and mutant ovarian carcinoma cells". Carcinogenesis . 22 (12): 1971–8.
doi :
10.1093/carcin/22.12.1971 .
PMID
11751427 .
^ Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (March 1999).
"Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes" . EMBO J . 18 (5): 1397–406.
doi :
10.1093/emboj/18.5.1397 .
PMC
1171229 .
PMID
10064605 .
^
a
b Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (April 2002).
"Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA" . EMBO J . 21 (7): 1855–63.
doi :
10.1093/emboj/21.7.1855 .
PMC
125950 .
PMID
11927569 .
^ Bochkareva E, Frappier L, Edwards AM, Bochkarev A (February 1998).
"The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain" . J. Biol. Chem . 273 (7): 3932–6.
doi :
10.1074/jbc.273.7.3932 .
PMID
9461578 .
^ Unsal-Kaçmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK (April 2007).
"The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement" . Mol. Cell. Biol . 27 (8): 3131–42.
doi :
10.1128/MCB.02190-06 .
PMC
1899931 .
PMID
17296725 .
^ Yoo E, Kim BU, Lee SY, Cho CH, Chung JH, Lee CH (August 2005).
"53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage" . Oncogene . 24 (35): 5423–30.
doi :
10.1038/sj.onc.1208710 .
PMID
15856006 .
^ Li L, Lu X, Peterson CA, Legerski RJ (October 1995).
"An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair" . Mol. Cell. Biol . 15 (10): 5396–402.
doi :
10.1128/mcb.15.10.5396 .
PMC
230789 .
PMID
7565690 .
Further reading
Iftode C, Daniely Y, Borowiec JA (1999). "Replication protein A (RPA): the eukaryotic SSB". Crit. Rev. Biochem. Mol. Biol . 34 (3): 141–80.
doi :
10.1080/10409239991209255 .
PMID
10473346 .
Binz SK, Sheehan AM, Wold MS (2005). "Replication protein A phosphorylation and the cellular response to DNA damage". DNA Repair (Amst.) . 3 (8–9): 1015–24.
doi :
10.1016/j.dnarep.2004.03.028 .
PMID
15279788 .
Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E (1992).
"Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen" . EMBO J . 11 (2): 769–76.
doi :
10.1002/j.1460-2075.1992.tb05110.x .
PMC
556510 .
PMID
1311258 .
Erdile LF, Heyer WD, Kolodner R, Kelly TJ (1991).
"Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication" . J. Biol. Chem . 266 (18): 12090–8.
doi :
10.1016/S0021-9258(18)99069-1 .
PMID
2050703 .
Li L, Lu X, Peterson CA, Legerski RJ (1995).
"An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair" . Mol. Cell. Biol . 15 (10): 5396–402.
doi :
10.1128/mcb.15.10.5396 .
PMC
230789 .
PMID
7565690 .
Keshav KF, Chen C, Dutta A (1995).
"Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex" . Mol. Cell. Biol . 15 (6): 3119–28.
doi :
10.1128/mcb.15.6.3119 .
PMC
230543 .
PMID
7760808 .
Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ (1994).
"High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3)" . Genomics . 20 (2): 249–57.
doi :
10.1006/geno.1994.1161 .
PMID
8020972 .
Gomes XV, Wold MS (1996). "Functional domains of the 70-kilodalton subunit of human replication protein A.". Biochemistry . 35 (32): 10558–68.
doi :
10.1021/bi9607517 .
PMID
8756712 .
Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L (1997). "Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA". Nature . 385 (6612): 176–81.
Bibcode :
1997Natur.385..176B .
doi :
10.1038/385176a0 .
PMID
8990123 .
S2CID
4288187 .
Amacker M, Hottiger M, Mossi R, Hübscher U (1997). "HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase". AIDS . 11 (4): 534–6.
PMID
9084803 .
Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997).
"The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme" . Mol. Cell. Biol . 17 (4): 1817–23.
doi :
10.1128/MCB.17.4.1817 .
PMC
232028 .
PMID
9121429 .
Loor G, Zhang SJ, Zhang P, Toomey NL, Lee MY (1998).
"Identification of DNA replication and cell cycle proteins that interact with PCNA" . Nucleic Acids Res . 25 (24): 5041–6.
doi :
10.1093/nar/25.24.5041 .
PMC
147130 .
PMID
9396813 .
Bochkareva E, Frappier L, Edwards AM, Bochkarev A (1998).
"The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain" . J. Biol. Chem . 273 (7): 3932–6.
doi :
10.1074/jbc.273.7.3932 .
PMID
9461578 .
Golub EI, Gupta RC, Haaf T, Wold MS, Radding CM (1999).
"Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA" . Nucleic Acids Res . 26 (23): 5388–93.
doi :
10.1093/nar/26.23.5388 .
PMC
148005 .
PMID
9826763 .
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (1999).
"Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes" . EMBO J . 18 (5): 1397–406.
doi :
10.1093/emboj/18.5.1397 .
PMC
1171229 .
PMID
10064605 .
Brosh RM, Orren DK, Nehlin JO, Ravn PH, Kenny MK, Machwe A, Bohr VA (1999).
"Functional and physical interaction between WRN helicase and human replication protein A." J. Biol. Chem . 274 (26): 18341–50.
doi :
10.1074/jbc.274.26.18341 .
PMID
10373438 .
Kim ST, Lim DS, Canman CE, Kastan MB (2000).
"Substrate specificities and identification of putative substrates of ATM kinase family members" . J. Biol. Chem . 274 (53): 37538–43.
doi :
10.1074/jbc.274.53.37538 .
PMID
10608806 .
Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (2000).
"Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity" . J. Biol. Chem . 275 (31): 23500–8.
doi :
10.1074/jbc.M001557200 .
hdl :
10026.1/10318 .
PMID
10825162 .
PDB gallery
1ewi : HUMAN REPLICATION PROTEIN A: GLOBAL FOLD OF THE N-TERMINAL RPA-70 DOMAIN REVEALS A BASIC CLEFT AND FLEXIBLE C-TERMINAL LINKER
1fgu : SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A
1jmc : SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420
1l1o : Structure of the human Replication Protein A (RPA) trimerization core
2b29 : N-terminal domain of the RPA70 subunit of human replication protein A.
2b3g : p53N (fragment 33-60) bound to RPA70N