| Glycoside hydrolase family 44 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | Glyco_hydro_44 | ||||||||
| Pfam | PF12891 | ||||||||
| Pfam clan | CL0058 | ||||||||
| CAZy | GH44 | ||||||||
| |||||||||
In molecular biology, glycoside hydrolase family 44 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families. [1] [2] [3] This classification is available on the CAZy web site, [4] [5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. [6] [7]
Glycoside hydrolase family 44 CAZY GH_44, formerly known as cellulase family J, includes enzymes with endoglucanase EC 3.2.1.4 and xyloglucanase EC 3.2.1.151 activities. The overall structure of enzymes in this family consists of a TIM-like barrel domain, a beta-sandwich domain and an active site with two glutamic acid residues, all of which are conserved between the endoglucanases and xyloglucanases in the family, with only minor differences. [8] [9]
References
- ^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. Bibcode: 1995PNAS...92.7090H. doi: 10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
- ^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi: 10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 (Pt 2): 695–6. doi: 10.1042/bj3160695. PMC 1217404. PMID 8687420.
- ^ "Home". CAZy.org. Retrieved 2018-03-06.
- ^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490-5. doi: 10.1093/nar/gkt1178. PMC 3965031. PMID 24270786.
- ^ "Glycoside Hydrolase Family 44". CAZypedia.org. Retrieved 2018-03-06.
- ^ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes". Glycobiology. 28 (1): 3–8. doi: 10.1093/glycob/cwx089. hdl: 21.11116/0000-0003-B7EB-6. PMID 29040563.
- ^ Kitago Y, Karita S, Watanabe N, Kamiya M, Aizawa T, Sakka K, Tanaka I (December 2007). "Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum". The Journal of Biological Chemistry. 282 (49): 35703–11. doi: 10.1074/jbc.M706835200. PMID 17905739.
- ^ Ariza A, Eklöf JM, Spadiut O, Offen WA, Roberts SM, Besenmatter W, Friis EP, Skjøt M, Wilson KS, Brumer H, Davies G (September 2011). "Structure and activity of Paenibacillus polymyxa xyloglucanase from glycoside hydrolase family 44". The Journal of Biological Chemistry. 286 (39): 33890–900. doi: 10.1074/jbc.M111.262345. PMC 3190823. PMID 21795708.
