In
molecular biology, XhoI is a type II
restriction enzymeEC that recognise the double-stranded
DNA sequence CTCGAG and cleaves after C-1.[1] Type II
restriction endonucleases (
EC) are components of
prokaryotic DNA restriction-modification mechanisms that protect the organism against invading foreign DNA. These site-specific deoxyribonucleases
catalyse the endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.