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TaqI is a restriction enzyme isolated from the bacterium Thermus aquaticus in 1978. [1] It has a recognition sequence of
5'TCGA 3'AGCT
and makes the cut
5'---T CGA---3' 3'---AGC T---5'
References
- ^ Sato, S. (February 1978). "A single cleavage of Simian virus 40 (SV40) DNA by a site specific endonuclease from Thermus aquaticus, Taq I". J. Biochem. 83 (2): 633–5. doi: 10.1093/oxfordjournals.jbchem.a131952. PMID 204628.
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