From Wikipedia, the free encyclopedia
RRNA small subunit pseudouridine methyltransferase Nep1 (
EC
2.1.1.260 , Nep1 , nucleolar essential protein 1 ) is an
enzyme with
systematic name S-adenosyl-L-methionine:18S rRNA (pseudouridine1191 -N1 )-methyltransferase .
[1]
[2]
[3] This enzyme
catalyses the following
chemical reaction
S-adenosyl-L-methionine +
pseudouridine 1191 in
yeast
18S rRNA
⇌
{\displaystyle \rightleftharpoons }
S-adenosyl-L-homocysteine + N1 -methylpseudouridine1191 in yeast 18S rRNA
This enzyme recognizes specific
pseudouridine residues (Psi) in
small subunits of ribosomal RNA based on the local RNA structure.
A point mutation in the ribosome biogenesis factor Nep1 impairs its nucleolar localisation and RNA binding and causes the Bowen-Conradi syndrome.
[4]
.
[5]
References
^ Taylor AB, Meyer B, Leal BZ, Kötter P, Schirf V, Demeler B, Hart PJ, Entian KD, Wöhnert J (March 2008).
"The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site" . Nucleic Acids Research . 36 (5): 1542–54.
doi :
10.1093/nar/gkm1172 .
PMC
2275143 .
PMID
18208838 .
^ Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kötter P, Engels JW, Heckel A, Karas M, Entian KD, Wöhnert J (April 2010).
"The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase" . Nucleic Acids Research . 38 (7): 2387–98.
doi :
10.1093/nar/gkp1189 .
PMC
2853112 .
PMID
20047967 .
^ Meyer B, Wurm JP, Kötter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wöhnert J, Entian KD (March 2011).
"The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA" . Nucleic Acids Research . 39 (4): 1526–37.
doi :
10.1093/nar/gkq931 .
PMC
3045603 .
PMID
20972225 .
^ Meyer B, Wurm JP, Kotter P, Leisegang MS, Schilling V, Buchhaupt M, Held M, Bahr U, Karas M, Heckel A, Bohnsack MT, Wöhnert J, Entian KD (2011).
"The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Psi 1191 in yeast 18S rRNA" . Nucleic Acids Res . 39 (4): 1526–37.
doi :
10.1093/nar/gkq931 .
PMC
3045603 .
PMID
20972225 . {{
cite journal }}
: CS1 maint: multiple names: authors list (
link )
^ Wurm JP, Meyer B, Bahr U, Held M, Frolow O, Kotter P, Engels JW, Heckel A, Karas M, Entian KD, Wöhnert J (2010).
"The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase" . Nucleic Acids Res . 38 (7): 2387–98.
doi :
10.1093/nar/gkp1189 .
PMC
2853112 .
PMID
20047967 . {{
cite journal }}
: CS1 maint: multiple names: authors list (
link )
External links
Activity Regulation Classification Kinetics Types