Lys-N is a metalloendopeptidase found in the mushroom Grifola frondosa that cleaves proteins on the amino side of lysine residues. [1]
Mass spectrometry
Lys-N is becoming a popular protease used for protein digestion in proteomics experiments.[ citation needed] The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra composed mostly of amino terminal peptide fragment ions. [3] This fragmentation pattern facilitates the applicability of these spectra for de novo peptide sequencing. [3]
See also
References
- ^ Nonaka T, Hashimoto Y, Takio K (July 1998). "Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies". Journal of Biochemistry. 124 (1): 157–62. doi: 10.1093/oxfordjournals.jbchem.a022074. PMID 9644258.
- ^ RCSB Protein Data Bank - RCSB PDB - 1G12 Structure Summary
- ^ a b Taouatas N, Drugan MM, Heck AJ, Mohammed S (May 2008). "Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase". Nature Methods. 5 (5): 405–7. doi: 10.1038/nmeth.1204. PMID 18425140. S2CID 28504546.
External links
- The MEROPS online database for peptidases and their inhibitors: M35.004
- Coverage on Genomeweb
- Enzyme entry at SWISS-PROT
