From Wikipedia, the free encyclopedia
Protein-coding gene in the species Homo sapiens
Inner centromere protein is a
protein that in humans is encoded by the INCENP
gene .
[5]
[6]
[7] It is a regulatory
protein in the
chromosome passenger
complex (CPC). It is involved in regulation of the
catalytic proteins
Aurora B and
Aurora C .
[8]
[9]
[10] It acts in association with two other
proteins -
Survivin and
Borealin . These
proteins form a tight three-helical bundle. The
N-terminal
domain of INCENP is the domain involved in formation of this three-helical bundle while its
C-terminal
domain is responsible for the interaction with Aurora B.
[11]
[10]
In mammalian cells, two broad groups of
centromere -interacting proteins have been described: constitutively binding
centromere proteins and 'passenger' (or transiently interacting) proteins.
[12] The constitutive proteins include
CENPA (centromere protein A),
CENPB ,
CENPC1 , and
CENPD .
The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the
cell cycle .
[13] These include
CENPE ;
MCAK ;
KID ; cytoplasmic dynein (e.g.,
DYNC1H1 ); CliPs (e.g.
CLIP1 ); and CENPF/mitosin (
CENPF ). The inner centromere proteins (INCENPs),
[5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of
mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During
telophase , the proteins are located within the
midbody in the intercellular bridge, where they are discarded after
cytokinesis .
[7]
[14]
Interactions
INCENP has been shown to
interact with
H2AFZ ,
[15]
Survivin
[16] and
CDCA8 .
[17] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related
kinase . This interaction has been implicated in the coordination of
chromosome segregation with
cell division in
yeast .
[18]
References
^
a
b
c
GRCh38: Ensembl release 89: ENSG00000149503 –
Ensembl , May 2017
^
a
b
c
GRCm38: Ensembl release 89: ENSMUSG00000024660 –
Ensembl , May 2017
^
"Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
a
b Earnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci . 98 (4): 443–61.
doi :
10.1242/jcs.98.4.443 .
PMID
1860899 .
^ Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma . 110 (2): 65–74.
doi :
10.1007/s004120100130 .
PMID
11453556 .
S2CID
13778759 .
^
a
b
"Entrez Gene: INCENP inner centromere protein antigens 135/155kDa" .
^ Sasai, Kaori; Katayama, Hiroshi; Hawke, David H; Sen, Subrata (2016-01-01).
"Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex" . PLOS ONE . 11 (6): e0157305.
Bibcode :
2016PLoSO..1157305S .
doi :
10.1371/journal.pone.0157305 .
ISSN
1932-6203 .
PMC
4917241 .
PMID
27332895 .
^ Li, Xiangyu; Sakashita, Gyosuke; Matsuzaki, Hideki; Sugimoto, Kenji; Kimura, Keiji; Hanaoka, Fumio; Taniguchi, Hisaaki; Furukawa, Koichi; Urano, Takeshi (2004-11-01).
"Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C" . The Journal of Biological Chemistry . 279 (45): 47201–47211.
doi :
10.1074/jbc.m403029200 .
ISSN
1083-351X .
PMID
15316025 .
^
a
b Honda, Reiko; Körner, Roman; Nigg, Erich A (2003-08-01).
"Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis" . Molecular Biology of the Cell . 14 (8): 3325–3341.
doi :
10.1091/mbc.e02-11-0769 .
ISSN
1939-4586 .
PMC
181570 .
PMID
12925766 .
^ Jeyaprakash, A. Arockia; Klein, Ulf R.; Lindner, Doris; Ebert, Judith; Nigg, Erich A.; Conti, Elena (2007-10-19).
"Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together" . Cell . 131 (2): 271–285.
doi :
10.1016/j.cell.2007.07.045 .
ISSN
0092-8674 .
PMID
17956729 .
S2CID
15199822 .
^ Choo, K. H. Andy (1997).
The centromere . Oxford [Oxfordshire]: Oxford University Press.
ISBN
978-0-19-857780-5 .
^ Earnshaw WC, Mackay AM (September 1994).
"Role of nonhistone proteins in the chromosomal events of mitosis" . FASEB J . 8 (12): 947–56.
doi :
10.1096/fasebj.8.12.8088460 .
PMID
8088460 .
S2CID
14062246 .
^ Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999).
"Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice" . Hum. Mol. Genet . 8 (7): 1145–55.
doi :
10.1093/hmg/8.7.1145 .
PMID
10369859 .
^ Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003).
"Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development" . EMBO J . 22 (7): 1599–607.
doi :
10.1093/emboj/cdg160 .
PMC
152904 .
PMID
12660166 .
^ Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001).
"INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis" . Curr. Biol . 11 (11): 886–90.
Bibcode :
2001CBio...11..886W .
doi :
10.1016/S0960-9822(01)00238-X .
PMID
11516652 .
^ Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004).
"Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle" . J. Cell Biol . 166 (2): 179–91.
doi :
10.1083/jcb.200404001 .
PMC
2172304 .
PMID
15249581 .
^ Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002).
"The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis" . Mol. Biol. Cell . 13 (4): 1132–43.
doi :
10.1091/mbc.01-07-0330 .
PMC
102257 .
PMID
11950927 .
Further reading
Ainsztein AM, Kandels-Lewis SE, Mackay AM, Earnshaw WC (1999).
"INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1" . J. Cell Biol . 143 (7): 1763–74.
doi :
10.1083/jcb.143.7.1763 .
PMC
2175214 .
PMID
9864353 .
Martineau-Thuillier S, Andreassen PR, Margolis RL (1999). "Colocalization of TD-60 and INCENP throughout G2 and mitosis: evidence for their possible interaction in signalling cytokinesis". Chromosoma . 107 (6–7): 461–70.
doi :
10.1007/s004120050330 .
PMID
9914378 .
S2CID
24935187 .
Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000).
"Shotgun sequencing of the human transcriptome with ORF expressed sequence tags" . Proc. Natl. Acad. Sci. U.S.A . 97 (7): 3491–6.
Bibcode :
2000PNAS...97.3491D .
doi :
10.1073/pnas.97.7.3491 .
PMC
16267 .
PMID
10737800 .
Wheatley SP, Kandels-Lewis SE, Adams RR, et al. (2001). "INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow". Exp. Cell Res . 262 (2): 122–7.
doi :
10.1006/excr.2000.5088 .
PMID
11139336 .
Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (2001).
"INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis" . Curr. Biol . 11 (11): 886–90.
Bibcode :
2001CBio...11..886W .
doi :
10.1016/S0960-9822(01)00238-X .
PMID
11516652 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2003).
"Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903.
Bibcode :
2002PNAS...9916899M .
doi :
10.1073/pnas.242603899 .
PMC
139241 .
PMID
12477932 .
Parra MT, Viera A, Gómez R, et al. (2003).
"Dynamic relocalization of the chromosomal passenger complex proteins inner centromere protein (INCENP) and aurora-B kinase during male mouse meiosis" . J. Cell Sci . 116 (Pt 6): 961–74.
doi :
10.1242/jcs.00330 .
PMID
12584241 .
Rangasamy D, Berven L, Ridgway P, Tremethick DJ (2003).
"Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development" . EMBO J . 22 (7): 1599–607.
doi :
10.1093/emboj/cdg160 .
PMC
152904 .
PMID
12660166 .
Honda R, Körner R, Nigg EA (2004).
"Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis" . Mol. Biol. Cell . 14 (8): 3325–41.
doi :
10.1091/mbc.E02-11-0769 .
PMC
181570 .
PMID
12925766 .
Wheatley SP, Henzing AJ, Dodson H, et al. (2004).
"Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo" . J. Biol. Chem . 279 (7): 5655–60.
doi :
10.1074/jbc.M311299200 .
PMID
14610074 .
Ota T, Suzuki Y, Nishikawa T, et al. (2004).
"Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5.
doi :
10.1038/ng1285 .
PMID
14702039 .
Gassmann R, Carvalho A, Henzing AJ, et al. (2004).
"Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle" . J. Cell Biol . 166 (2): 179–91.
doi :
10.1083/jcb.200404001 .
PMC
2172304 .
PMID
15249581 .
Li X, Sakashita G, Matsuzaki H, et al. (2004).
"Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C" . J. Biol. Chem . 279 (45): 47201–11.
doi :
10.1074/jbc.M403029200 .
PMID
15316025 .
Gerhard DS, Wagner L, Feingold EA, et al. (2004).
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)" . Genome Res . 14 (10B): 2121–7.
doi :
10.1101/gr.2596504 .
PMC
528928 .
PMID
15489334 .
Zhu C, Bossy-Wetzel E, Jiang W (2005).
"Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells" . Biochem. J . 389 (Pt 2): 373–81.
doi :
10.1042/BJ20050097 .
PMC
1175114 .
PMID
15796717 .
Chen HL, Tang CJ, Chen CY, Tang TK (2005). "Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy". J. Biomed. Sci . 12 (2): 297–310.
doi :
10.1007/s11373-005-0980-0 .
PMID
15917996 .
Vader G, Kauw JJ, Medema RH, Lens SM (2006).
"Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody" . EMBO Rep . 7 (1): 85–92.
doi :
10.1038/sj.embor.7400562 .
PMC
1369225 .
PMID
16239925 .
Goto H, Kiyono T, Tomono Y, et al. (2006). "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition". Nat. Cell Biol . 8 (2): 180–7.
doi :
10.1038/ncb1350 .
PMID
16378098 .
S2CID
11583385 .