From Wikipedia, the free encyclopedia
Class of enzymes
Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (
EC
2.4.1.288 , GlfT2 ) is an
enzyme with
systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase .
[1]
[2]
[3] This enzyme
catalyses the following
chemical reaction
28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
⇌
{\displaystyle \rightleftharpoons }
28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
This enzyme is isolated from
Mycobacterium tuberculosis .
References
^ Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL (August 2008). "Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase". Carbohydrate Research . 343 (12): 2130–9.
doi :
10.1016/j.carres.2008.03.023 .
PMID
18423586 .
^ May JF, Splain RA, Brotschi C, Kiessling LL (July 2009).
"A tethering mechanism for length control in a processive carbohydrate polymerization" . Proceedings of the National Academy of Sciences of the United States of America . 106 (29): 11851–6.
Bibcode :
2009PNAS..10611851M .
doi :
10.1073/pnas.0901407106 .
PMC
2715488 .
PMID
19571009 .
^ Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK (August 2012).
"Tetrameric structure of the GlfT2 galactofuranosyltransferase reveals a scaffold for the assembly of mycobacterial Arabinogalactan" . The Journal of Biological Chemistry . 287 (33): 28132–43.
doi :
10.1074/jbc.M112.347484 .
PMC
3431634 .
PMID
22707726 .
External links
Activity Regulation Classification Kinetics Types