From Wikipedia, the free encyclopedia
Dihydroorotate dehydrogenase (
fumarate ) (
EC
1.3.98.1 , dihydroorotate oxidase, pyr4 (gene) ) is an
enzyme with
systematic name (S)-dihydroorotate:fumarate oxidoreductase .
[1]
[2]
[3]
[4]
[5]
[6] This enzyme
catalyses the following
chemical reaction
(S)-
dihydroorotate +
fumarate
⇌
{\displaystyle \rightleftharpoons }
orotate +
succinate
This enzyme contains FMN.
References
^ Björnberg O, Rowland P, Larsen S, Jensen KF (December 1997). "Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis". Biochemistry . 36 (51): 16197–205.
doi :
10.1021/bi971628y .
PMID
9405053 .
^ Rowland P, Björnberg O, Nielsen FS, Jensen KF, Larsen S (June 1998).
"The crystal structure of Lactococcus lactis dihydroorotate dehydrogenase A complexed with the enzyme reaction product throws light on its enzymatic function" . Protein Science . 7 (6): 1269–79.
doi :
10.1002/pro.5560070601 .
PMC
2144028 .
PMID
9655329 .
^ Nørager S, Arent S, Björnberg O, Ottosen M, Lo Leggio L, Jensen KF, Larsen S (August 2003).
"Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function" . The Journal of Biological Chemistry . 278 (31): 28812–22.
doi :
10.1074/jbc.M303767200 .
PMID
12732650 .
^ Zameitat E, Pierik AJ, Zocher K, Löffler M (September 2007).
"Dihydroorotate dehydrogenase from Saccharomyces cerevisiae: spectroscopic investigations with the recombinant enzyme throw light on catalytic properties and metabolism of fumarate analogues" . FEMS Yeast Research . 7 (6): 897–904.
doi :
10.1111/j.1567-1364.2007.00275.x .
PMID
17617217 .
^ Inaoka DK, Sakamoto K, Shimizu H, Shiba T, Kurisu G, Nara T, Aoki T, Kita K, Harada S (October 2008). "Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction". Biochemistry . 47 (41): 10881–91.
doi :
10.1021/bi800413r .
PMID
18808149 .
^ Cheleski J, Wiggers HJ, Citadini AP, da Costa Filho AJ, Nonato MC, Montanari CA (April 2010). "Kinetic mechanism and catalysis of Trypanosoma cruzi dihydroorotate dehydrogenase enzyme evaluated by isothermal titration calorimetry". Analytical Biochemistry . 399 (1): 13–22.
doi :
10.1016/j.ab.2009.11.018 .
PMID
19932077 .
External links
Activity Regulation Classification Kinetics Types