From Wikipedia, the free encyclopedia
DNA oxidative demethylase (
EC
1.14.11.33 , alkylated DNA repair protein , alpha-ketoglutarate-dependent dioxygenase ABH1 , alkB (gene) ) is an
enzyme with
systematic name methyl DNA-base, 2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming) .
[1]
[2]
[3] This enzyme
catalyses the following
chemical reaction
DNA-base-CH3 +
2-oxoglutarate + O2
⇌
{\displaystyle \rightleftharpoons }
DNA-base +
formaldehyde +
succinate + CO2
DNA oxidative demethylase contains
iron ; activity is somewhat stimulated by
ascorbate .
References
^ Falnes PØ, Johansen RF, Seeberg E (September 2002). "AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli". Nature . 419 (6903): 178–82.
Bibcode :
2002Natur.419..178F .
doi :
10.1038/nature01048 .
PMID
12226668 .
S2CID
2372162 .
^ Yi C, Yang CG, He C (April 2009).
"A non-heme iron-mediated chemical demethylation in DNA and RNA" . Accounts of Chemical Research . 42 (4): 519–29.
doi :
10.1021/ar800178j .
PMC
2920458 .
PMID
19852088 .
^ Yi C, Jia G, Hou G, Dai Q, Zhang W, Zheng G, Jian X, Yang CG, Cui Q, He C (November 2010).
"Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase" . Nature . 468 (7321): 330–3.
Bibcode :
2010Natur.468..330Y .
doi :
10.1038/nature09497 .
PMC
3058853 .
PMID
21068844 .
External links
Activity Regulation Classification Kinetics Types