Cannabidiolic acid synthase | |||||||||
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Identifiers | |||||||||
EC no. | 1.21.3.8 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Cannabidiolic acid synthase ( EC 1.21.3.8, CBDA synthase) is an enzyme with systematic name cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming). [1] [2] It is an oxidoreductase found in Cannabis sativa that catalyses the formation of cannabidiolate, a carboxylated precursor of cannabidiol. [2]
Cannabidiolic acid synthase consists of a single protein with a molecular mass of 74 kDa. [1] Its amino acid sequence is partly (40-50%) homologous to several other oxidoreductases, [2] such as berberine bridge enzyme in Eschscholzia californica [3] and Nectarin V in Nicotiana langsdorffii X N. sanderae. [4]
CBDA synthase has four binding sites; two for FAD and two for the substrate. [5]
Cannabidiolic acid synthase catalyses the production of cannabidiolate predominantly from cannabigerolate by stereospecific oxidative cyclization of the geranyl group of cannabigerolic acid [2] according to the following chemical reaction:
Cannabinerolate can also be used as a substrate, but with lower efficiency ( KM=0.137 mM) than cannabigerolate (KM=0.206 mM). It covalently binds FAD, and does require coenzymes & molecular oxygen for the oxidocyclization reaction. [1]
The optimum pH for CBDA synthase is 5.0. [2]