From Wikipedia, the free encyclopedia
Class of enzymes
23S rRNA (guanine2535 -N1 )-methyltransferase (
EC
2.1.1.209 , AviRa ) is an
enzyme with
systematic name S-adenosyl-L-methionine:23S rRNA (guanine253 5-N1 )-methyltransferase .
[1]
[2]
[3] This enzyme
catalyses the following
chemical reaction
S-adenosyl-L-methionine +
guanine 2535 in
23S rRNA
⇌
{\displaystyle \rightleftharpoons }
S-adenosyl-L-homocysteine + N1 -methylguanine2535 in 23S rRNA
This is one of the
methyltransferases from
Streptomyces viridochromogenes .
Streptomyces viridochromogenes produces the
antibiotic
avilamycin A which binds to the
50S ribosomal subunit to inhibit
protein synthesis .
[4]
References
^ Treede I, Jakobsen L, Kirpekar F, Vester B, Weitnauer G, Bechthold A, Douthwaite S (July 2003).
"The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose" . Molecular Microbiology . 49 (2): 309–18.
doi :
10.1046/j.1365-2958.2003.03558.x .
PMID
12828631 .
^ Weitnauer G, Gaisser S, Trefzer A, Stockert S, Westrich L, Quiros LM, Mendez C, Salas JA, Bechthold A (March 2001).
"An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tü57" . Antimicrobial Agents and Chemotherapy . 45 (3): 690–5.
doi :
10.1128/aac.45.3.690-695.2001 .
PMC
90357 .
PMID
11181344 .
^ Mosbacher TG, Bechthold A, Schulz GE (May 2003). "Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes". Journal of Molecular Biology . 329 (1): 147–57.
doi :
10.1016/s0022-2836(03)00407-8 .
PMID
12742024 .
^ Krupkin M, Wekselman I, Matzov D, Eyal Z, Diskin Posner Y, Rozenberg H, Zimmerman E, Bashan A, Yonath A (November 2016).
"Avilamycin and evernimicin induce structural changes in rProteins uL16 and CTC that enhance the inhibition of A-site tRNA binding" . Proceedings of the National Academy of Sciences of the United States of America . 113 (44): E6796–E6805.
doi :
10.1073/pnas.1614297113 .
PMC
5098648 .
PMID
27791159 .
External links
Activity Regulation Classification Kinetics Types