From Wikipedia, the free encyclopedia
Y-27632
Names
IUPAC name
(1R ,4r )-4-((R )-1-Aminoethyl)-N -(pyridin-4-yl)cyclohexanecarboxamide
Identifiers
ChEBI
ChEMBL
ChemSpider
DrugBank
ECHA InfoCard
100.149.247
UNII
InChI=1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11-,12-/m1/s1
Y Key: IYOZTVGMEWJPKR-IJLUTSLNSA-N
Y InChI=1/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11-,12-/m1/s1
Key: IYOZTVGMEWJPKR-IJLUTSLNBP
O=C([C@H]1CC[C@@]([C@H](N)C)([H])CC1)NC2=CC=NC=C2
Properties
C 14 H 21 N 3 O
Molar mass
247.342 g·mol−1
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
Chemical compound
Y-27632 is a biochemical tool used in the study of the
rho-associated protein kinase (ROCK) signaling pathways.
[1] Y-27632 selectively inhibits
p160ROCK , although it does inhibit other protein kinases such as
PKCs at higher concentrations.
[2]
It has been studied for its effects on corneal
endothelial cells (CECs)
[3] and cardiac
stem cells (CSCs).
[4]
The substance has been used as part of a chemical cocktail to turn old and
senescent human cells back into young ones (as measured by transcriptomic age), without turning them all the way back into undifferentiated
stem cells .
[5]
A mixture of
methylcellulose (1%),
ethylene glycol (10%),
dimethyl sulfoxide (10%), and Y-27632 (10 μM), termed MEDY, has been shown to be an effective
cryopreservation reagent for brain tissue, enabling tissue to resume growth and function after freezing using liquid nitrogen.
[6]
References
^ Uehata, Masayoshi (1999). "Y-27632. Selective probe of ROCK/Rho-kinase". Jikken Igaku . 17 (7): 850–855.
^ Uehata, M; Ishizaki, T; Satoh, H; Ono, T; Kawahara, T; Morishita, T; Tamakawa, H; Yamagami, K; et al. (1997). "Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension". Nature . 389 (6654): 990–4.
Bibcode :
1997Natur.389..990U .
doi :
10.1038/40187 .
PMID
9353125 .
S2CID
4419556 .
^ Peh, Gary S. L.; Adnan, Khadijah; George, Benjamin L.; Ang, Heng-Pei; Seah, Xin-Yi; Tan, Donald T.; Mehta, Jodhbir S. (2015-03-16).
"The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach" . Scientific Reports . 5 : 9167.
Bibcode :
2015NatSR...5E9167P .
doi :
10.1038/srep09167 .
ISSN
2045-2322 .
PMC
4387913 .
PMID
25823914 .
^ Kan, Lijuan; Smith, Aubrie; Chen, Miao; Ledford, Benjamin T.; Fan, Huimin; Liu, Zhongmin; He, Jia-Qiang (2015-12-08).
"Rho-Associated Kinase Inhibitor (Y-27632) Attenuates Doxorubicin-Induced Apoptosis of Human Cardiac Stem Cells" . PLOS ONE . 10 (12): e0144513.
Bibcode :
2015PLoSO..1044513K .
doi :
10.1371/journal.pone.0144513 .
ISSN
1932-6203 .
PMC
4672899 .
PMID
26645568 .
^ Yang, Jae-Hyun; Petty, Christopher A.; Dixon-McDougall, Thomas; Lopez, Maria Vina; Tyshkovskiy, Alexander; Maybury-Lewis, Sun; Tian, Xiao; Ibrahim, Nabilah; Chen, Zhili; Griffin, Patrick T.; Arnold, Matthew; Li, Jien; Martinez, Oswaldo A.; Behn, Alexander; Rogers-Hammond, Ryan (2023-07-12).
"Chemically induced reprogramming to reverse cellular aging" . Aging . 15 (13): 5966–5989.
doi :
10.18632/aging.204896 .
ISSN
1945-4589 .
PMC
10373966 .
PMID
37437248 .
^ Xue, Weiwei; Li, Huijuan; Xu, Jinhong; Yu, Xiao; Liu, Linlin; Liu, Huihui; Zhao, Rui; Shao, Zhicheng (2024).
"Effective cryopreservation of human brain tissue and neural organoids" . Cell Reports Methods . 4 (5).
doi :
10.1016/j.crmeth.2024.100777 .
PMC
11133841 .
PMID
38744289 .