From Wikipedia, the free encyclopedia
Tre recombinase is an experimental
enzyme that in lab tests has removed
DNA inserted by
HIV from infected cells.
[1] Through
selective mutation,
Cre recombinase which recognizes loxP sites are modified to identify HIV
long terminal repeats (loxLTR) instead. As a result, instead of performing
Cre-Lox recombination, the new enzyme performs recombination at HIV
provirus sites.
[2]
The structure of Tre in complex with loxLTR has been resolved (
PDB:
5U91), allowing for analyzing the roles of individual mutations.
[3]
References
-
^ Sarkar, Indrani; Hauber, Ilona; Hauber, Joachim; Buchholz, Frank (2007). "HIV-1 proviral DNA excision using an evolved recombinase".
Science. 316 (5833): 1912–15.
Bibcode:
2007Sci...316.1912S.
doi:
10.1126/science.1141453.
PMID
17600219.
S2CID
2437602.
-
^ Hauber, Ilona; Hofmann-Sieber, Helga; Chemnitz, Jan; et al. (September 26, 2013).
"Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice". PLOS Pathogens. 9 (9): e1003587.
doi:
10.1371/journal.ppat.1003587.
PMC
3784474.
PMID
24086129.
-
^ Meinke, G; Karpinski, J; Buchholz, F; Bohm, A (19 September 2017).
"Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA". Nucleic Acids Research. 45 (16): 9726–9740.
doi:
10.1093/nar/gkx603.
PMC
5766204.
PMID
28934476.
External links