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How does "scFv" correspond to "Single Chain Variable Fragment"? Google shows that "Single Chain Fragment Variable" is also in use, isn't that more accurate? —Preceding unsigned comment added by 84.14.90.154 ( talk) 07:32, 4 September 2008 (UTC)
Most of the publications suggesting that the affinity of diabodies can be increased by a factor of 40 are very poorly performed or even wrong. The reason is that most reviewers don't understand binding kinetics and researches doing SPR often also not. Everything beyond a total KD of 1/KD = 1/KD1 + 1/KD2 + .. is likely due to rebinding and very bad data fitting. I am getting sick when I read crap like this. It is just not physically possible to achieve these rates. There is even a nice book article explaining the theoretical maximum KD of multivalent analytes: Krishnamurthy VM, Estroff LA, Whitesides GM. Multivalency in Ligand Design. Fragment-based Approaches in Drug Discovery: Wiley-VCH Verlag GmbH & Co. KGaA; 2006. p. 11-53. — Preceding unsigned comment added by 194.94.44.220 ( talk) 09:59, 20 April 2015 (UTC)