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This is an interesting article addressing why silent mutations might not be so silent. [ [1]]
If there are parrallel codes in genomic sequences then altering one base pair, while not changing the actual amino acid, might impact other lines of coded information; possibly information pertaining to gene regulation, splicing of pre-mRNA. The more we learn about DNA the more amazing it becomes. --jorgekluney
Hello author of article, You might want to link some of the studies that have been finding dual-coding genes. Talk about exciting! Further research showing that there might be alternate reading-frames in the same genetic segment (overlapping genes *in the same segment* that code for multiple proteins-wow). --jorgekluney
On the other hand, the degree of evolutionary sequence conservation is higher for proteins than for the DNA encoding them, indicating that many or most silent mutations within reading frames are, in fact, silent.
This article was the subject of a Wiki Education Foundation-supported course assignment, between 27 August 2018 and 7 December 2018. Further details are available
on the course page. Student editor(s):
Demeanazarea.
Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT ( talk) 09:21, 17 January 2022 (UTC)
The article is an important topic in molecular biology and genetics, and thus should be expanded to further introduce and explain the topic. Expansion to the introductory section is planned including refining what is already submitted, including adding an image to better illustrate the definition of "silent mutation", and adding further background or history as applicable. Current discoveries or research updates will be added as well to illustrate the relevance of the topic. A more comprehensive tie in with the degeneracy of the genetic code can help to further develop the topic along with discussing the role of silent mutations in protein structure, folding, and function from the primary level up to tertiary and quaternary levels. The role of silent mutations in disease will be expanded with additional examples given. Finally, the experimental use of silent mutations in research and biotechnilogical advancements will be examined. Awotawa1 ( talk) 03:00, 14 March 2013 (UTC)
So far, good contributions to this article. I would like to suggest cutting your lead section down. The first paragraph does a nice job summing up the article and I think the second paragraph adds some good information as well that could be kept. However, the second paragraph (if you want) and definitely the third could be made into their own sections if you had more to add to it. A section on studies performed such as what you mentioned about molecular cloning would be a great addition or you could simply add this to your examples section.
Another good contribution to this article would be visuals. An image of codons and the wobble position would be good in the section on silent mutations and genetic code. Also a chart of codon synonyms would be a good display of your content here.
Sources need to be cited in the tRNA section as well as in the examples section. I am confused by your references list at the bottom. Maybe these are what are supposed to be the sources for the two sections but it does not follow the format that allows readers to know exactly where your information came from.
Also, the nonsynonymous link does not exist in the examples section. There is a nonsynonymous substitution article you could link. However, you will notice that this is also a stub article so a better choice may be more appropriate. Jberendt ( talk) 03:14, 11 April 2013 (UTC)
Since all of these articles are in their infancy essentially, it seems rather unhelpful to critique the lack of detail or precise syntax. I understand the authors already know this. Therefore, my critique focuses on providing feedback concerning definitions, focus, and structure.
Generally, I like the way this article is structured. My sampling of the references indicated they are properly placed and lend great credibility to the assertions made. Although this article currently has no image or cartoon demonstrating the concept of a silent mutation, some concepts simply are not very amenable to pictorial representation. Pictures can sometimes confuse more than they teach and that may be the case with silent mutation. I have just one major critique of this article and because any advice regarding structure is somewhat dependent on how the authors respond to it, I will await the response.
There is one basic definitional problem within this article that needs to be clearly resolved in order to avoid confusion going forward. The authors point out that the term ‘silent mutation’ is used broadly to encompass non-consequential mutations as well as synonymous mutations. I do not doubt this is the case, but the authors have to be wary of maintaining this distinction. For instance, in the first sentence of the lead section silent mutation means one of two things: either a synonymous point mutation or an actual substitution with no discernible consequence. However, in the section further down titled ‘Structural Consequences of Silent Mutations’, silent mutation means only the former.
This distinction might be clear in the authors' heads, but I was confused when I first encountered the last sentence of the lead section. If the definition of a silent mutation is essentially any mutation with no discernible consequence, then a mutation with a definite and measurable affect should not be a gradation or a subset of a silent mutation. After reviewing the footnotes provided for this sentence, I understood the source of my confusion. The Beckman reference is using the synonymous mutation definition of silent mutation, a narrower definition than the wiki article lead. Beckman defines silent mutation strictly as a point mutation with no change in amino acid sequence. With this narrower definition, it makes sense to say that a silent mutation is not necessarily so silent if, in fact, there are still consequences. On the other hand, using the broader definition offered by the wiki article lead section creates a fundamental contradiction. The wiki article might very well be correct in describing the term’s use more broadly, but if so, the different senses of ‘silent mutation’ need to be carefully distinguished early on to avoid any confusion. Jakodak ( talk) 15:36, 11 April 2013 (UTC)
Hi guys, I've got a full review but I need to make something very clear before I start. Plagiarism (or as we call it here, copyright violation) is totally and completely unacceptable. I found three separate instances of blatant plagiarism in this article and removed them all. I've asked you in the review below to rewrite that information without copying it. Plagiarizing here is not just an issue of academic integrity, it is a very serious legal issue for Wikipedia, and besides, continuing to plagiarize can get you blocked. Please be more careful in the future. With that said, here's a full review. Keilana| Parlez ici 21:59, 19 April 2013 (UTC)
Awotawa1 and Tbrownc1, your article is starting to come together quite well and has a nice flow to it. Wonderful job! I look forward to seeing the final product next week! With that being said, please see my suggestions listed below, and do not feel obligated to make these changes.
I look forward to the final contributions next week. If either of you have any questions and/or comments that I can help you both with, please feel free to let me know. Good luck! Jbmontgomery24 ( talk) 18:12, 1 May 2013 (UTC)
Hi guys, Overall I think that the article is good. I agree with everything that jbmontgomery24 says. I think that it flows pretty well but it definitely needs images. I really like the Examples section, but I don't think I like that title. I saw the title and thought I was going to see examples of silent mutations. Maybe name it Effects of Silent mutations or something like that? My only suggestion that hasn't been touched on by others is in regards to the "Tertiary structure" section. I know we are all walking a fine line between too little and too much information on our pages. This section seems light to me. Another sentence or two, or even an example will help. I look forward to seeing the final product next week! Laurenwking ( talk) 03:34, 3 May 2013 (UTC)
I have to edit this article for my genetics class so if anyone has any tips or anything they think I should add to make the article better just let me know! Rachelv37 ( talk) 22:50, 30 September 2018 (UTC)
This article has well written facts and explantation on silent mutations. The introduction is excellent. It has very broad and gives a good background knowledge of what silent mutations are. One thing that should be added in this article is information on mutations in other RNAs. For example, add in information about mRNA or rRNA. The article is very neutral although it needs a little bit more information about types of RNA. — Preceding unsigned comment added by Yp2h ( talk • contribs) 18:30, 13 October 2018 (UTC)
An amino acid chain can be made of hundred of amino acids, each with specific roles. The chain has a three-dimensional shape and folds over on itself. [1] The production rate of the nucleotides is speed dependent and any type of variation in the speed can cause the protein’s final form to be quite different. [1] Most cells can accept one silent mutation and function properly. If there are two silent mutations or more on the same amino acid chain, the cell may not function properly or at all. [1] Having more than one silent mutation on a single amino acid chain is very rare but can still happen. A silent mutation is a mutation in which the base sequence has been altered on the codon. [2] A silent mutation affects the gene by substituting on the base of the gene. [3] The base sequence codes nucleotides in sets of three, in which each of them is responsible for adding a specific amino acid to the protein chain. [1] A change in only one of the nucleotide bases does not always change the meaning of the protein. This means the sequence of the amino acid of the polypeptide remains unchanged. [4] With the amino acid unchanged, the protein remains functional [2]. Silent mutations are not likely to produce a polypeptide that reduced the proteins function. [4] Sometimes a silent mutation can change the polypeptide and enhance the function of the protein which would show no effect in the overall function. Other times silent mutations can alter the polypeptide and can decrease functioning of the protein which can either be unnoticed or can cause diseases. One example of a diseased caused from a silent mutation is familial hypercholesterolemia. Familial hypercholesterolemia, FH, is a disorder that is genetically inherited. [5] FH is characterized by an increased level in the Low-density lipoprotein in our cholesterol. FH contains a lot of mutations in the Low-density lipoprotein receptor gene. This results in having a lack of functioning receptors on the surface of the liver for the Low-density lipoprotein. [5] This allows for an increase in the plasma Low-density lipoprotein. Most mutations are rare and have no effect on the protein or over all functioning of a person. In the Low-density lipoprotein however, this is not true. To find out if someone has Low-density lipoprotein or other silent mutations, doctors can use a mutation scanning. MeltMADGE is a screening that is done in a blind trials. [6] This screening uses electrophoresis to in which amplicons are used to represent the coding region of the gene. By using this panel and known cholesterol values, the researches were able to identify a mutation, which is associated with hypercholesteremia, a silent mutation. They were also able to find splice mutations on the gene as well. [6] From using this screening and other resources like this, researchers can figure out how rare silent mutations are and what diseases are more commonly associated with them.
References
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was invoked but never defined (see the
help page).I moved this from the lead, because this isn't an overview of what's in the article. I think there's some useful stuff in there, but I'm not quite sure how it would fit in. Ian (Wiki Ed) ( talk) 15:49, 19 November 2018 (UTC)
@ AnnMarieOwens1993, Demeanazarea, Rachelv37, Ashleybrooksmtsu, and Riya Patel98: I removed a lot of your work, because it doesn't conform to the requirements of a Wikipedia article. A Wikipedia article is supposed to be about a single topic - in this case, silent mutations. You shouldn't be adding general information about tRNA or the genetic code to this article - general like that belongs in the transfer RNA or genetic code articles. Please feel free to get in touch with me if you have more questions. Ian (Wiki Ed) ( talk) 15:54, 19 November 2018 (UTC)