Proteasome is a former featured article. Please see the links under Article milestones below for its original nomination page (for older articles, check the nomination archive) and why it was removed. | ||||||||||||||||
This article appeared on Wikipedia's Main Page as Today's featured article on November 23, 2010. | ||||||||||||||||
| ||||||||||||||||
Current status: Former featured article |
This
level-5 vital article is rated B-class on Wikipedia's
content assessment scale. It is of interest to the following WikiProjects: | ||||||||||||||
|
This article was the
MCB Collaboration of the Month for the month of November 2006. For more details, see the
MCB Collaboration of the Month history. |
Although the proteasome article does include information regarding the ubiquitin proteasome system, it is important to recognize that the proteasome is only the final component of the ubiquitin proteasome system. The ubiquitin proteasome system is extremely extensive and has a high relevance to many areas of biomedical science e.g. cancer. Therefore I believe it is justified for this article exist in it's own right, but in a much more extensive form than at present.
Pharmacological information should relate primarily to bortezomib, the proteasome inhibitor used for multiple myeloma. JFW | T@lk 12:32, 1 November 2006 (UTC)
Motivated by the impressive diagrams of WikipedianProlific I'd like to have a go, too and draw one for the ubiquitin-proteasome-pathway. What steps do you think we should include (maybe starting from ubiquitination...) and do you know any good reviews about the topic? -- Splette :) Talk 23:31, 2 November 2006 (UTC)
I think that this article is about ready for GA nomination... what do you all think? – Clockwork Soul 15:00, 2 December 2006 (UTC)
Ummm, my initial impression is that the article is wonderfully complete; but it could be daunting for a typical lay-reader, who might benefit from a rough sketch of the structure and function early on. Maybe you agree? If I were a novice reader (not too far off ;), I think I might have an easier time reading more like this:
This is just a sketch and, since I'm going from memory, I'm not even sure whether it's accurate! It's just meant to give you an idea of the level that I think might be more comfortable for many non-expert readers. If you all think it'd be helpful, I'd be happy to make a 2D (subunits=circles) or 3D (subunits=spheres) schematic Figure of the 20S proteasome and the poly-Ub tag.
I'll look the article over later again today and see if I have any other ideas/suggestions; this was just a cursory look. Willow 16:24, 6 December 2006 (UTC)
OK, I'll try to make a decent schematic sometime today. Do you have any special preferences for colors or layout? My initial thought is to represent the proteasome by 3D spheres located at the centroid of each subunit, perhaps using the same colors as you do in the ribbon diagram? A cutaway diagram might be cool, too, say, by removing two subunits from each ring so that the reader could look inside and see the catalytic threonines — they're found in the crystal structure, I hope, not too floppy/disordered? Oooh! cooler yet, how about modeling in a hapless polypeptide in the throes of its destruction? I'll fool around and try to make a diagram that's instructive, yet respectful of the moritura.
Re:the lead section, I'm still worried that it may confound even smart lay-people, or at least make for slow reading, since they'll have to look up what "sub-unit", "complex", "protease", etc. means. Here I feel an awful pang of conscience, since Laplace-Runge-Lenz vector is far worse for obscurity. :( — BTW, thanks very much for your excellent insights/comments over there; it's so helpful to have an extra five non-physicist/mathematician eyes look it over! :) — Still, although my own articles fall short, I would warmly encourage you to make the lead section even more accessible to those smart lay-people who may not know the terminology but wish to form a picture of the proteasome, what it's doing, and why. I sometimes think of a much-cherished aunt, but you could imagine writing for a fondly remembered history professor, a hip high-school physics teacher, a nice doctor, a savvy science reporter, or, say, a judge deciding a patent case involving proteasomes. Willow 12:16, 7 December 2006 (UTC)
Rather than locating a scanner, I thought you might appreciate a Figure or three:
They're described in more detail on their respective pages on the Commons. I had lots of fun making them — the proteasome is so beautiful!
Are these Figures kind of what you wanted? The third one is cute, but might need more refinement? I can fix them up however you'd like. Dream big, for magic abounds. ;) Willow 23:52, 12 December 2006 (UTC)
MOLMOL does indeed have fog depth-cueing and I succeeded in shrinking the atoms down to a more decorous 3 Å. But looking at image #3 and its successors more closely, I noticed that some of the grey "loop" ribbons seemed disconnected, which I don't understand and can't seem to fix. :( So, I'm thinking of trying to re-make the Figure in PyMOL, with which I have only the vaguest experience. (Willow hears the beating of mighty wings as she rushes in. — "What can that be?" she wonders. "It sounds familiar." ;) Can you wait until tomorrow sometime? I'll do my best. :) Willow 23:23, 13 December 2006 (UTC)
Is it really an organelle? I don't think of it that way, and the closest text I had on hand gives it the next classification down - "molecular machine" - which is not really specific enough to get its own category. Opabinia regalis 03:31, 19 December 2006 (UTC)
Should "ubiquitin proteasome pathway" be hyphenated (i.e., ubiquitin-proteasome pathway)? A quick Google search suggests so; I have also frequently seen it hyphenated in Portuguese. Fvasconcellos 15:04, 3 January 2007 (UTC)
Does anybody know something about him? He discovered it... — Preceding unsigned comment added by 98.216.105.235 ( talk) 03:13, 11 August 2011 (UTC)
I added a little information on how there are different types of ubiquitin chains, and that only a subset of these chains will target proteins to the proteasome Maximus155 ( talk) 00:43, 3 February 2013 (UTC)
A newer review article talks a lot about the E3 ligases. Not sure if its needs its own page or a least add some of the names mentioned in the article. The article can be found at http://www.ncbi.nlm.nih.gov/pubmed?term=the%20ubiquitin-proteasome%20system%3A%20central%20modifier%20of%20plant%20signalling BreCaitlin ( talk) 01:36, 4 February 2013 (UTC)
I'm fairly new to using wikipedia, and until this article, I have not uploaded any images. However, there is a great image in a review article: http://www.ncbi.nlm.nih.gov/pubmed/23029643 that details the several types of proteasomes that exist. Although I realize that there is the potential for a copyright infringement, I uploaded the photo to Commons Wikipedia and inserted it into the article under "Role in the Immune System." Unfortunately, I did not know what copyright tag to use on the photo and so I put it as "fairuse" (which I know is not allowed). I did this hoping that someone would be able to clarify the issue and put the correct tag on it and/or delete it. However, since I found the article online (NCBI) and it is available to the public, I was assuming the copyright permission for redistribution was also allowed. Would someone be willing to respond and tell me what the criteria are for images in scholarly articles?
Also, if it turns out the image I did use is in violation of copyright law and must be removed, I still think that adding an image to show the several types of proteasomes and their components would be ideal. Therefore, maybe someone would be willing to create a diagram or cartoon of this? Thanks! MChapman5 ( talk) 02:02, 4 February 2013 (UTC)
There is new research [4] that shows that the immunoproteasome has another function outside of processing proteins for MHC I. It demonstrates that the immunoproteasome also helps to prevent apoptosis during IFN-induced oxidative stress. I want to include this information in this article, but I am not sure whether it should be included in the Apoptosis or Response to Cellular Stress section. Gpruett2 ( talk) 04:38, 4 February 2013 (UTC)
There are several references made the S26 Proteasome, but none of them out right say that they are only found in Eukaryotes. I would like to add this in one place based upon the following reference [1] As I am a new Wikipedian I would like some feedback from more senior members. — Preceding unsigned comment added by Hakkinen2013 ( talk • contribs) 17:32, 4 February 2013 (UTC)
References
I propose that a section be added to explain some of the laboratory uses of ubiquitin. For example, ubiquitin can be used to create an N-terminal fusion with your Protein of Interest (N-Ub-POI-C). This fusion protein could be transformed/transfected into a cell for expression (plasmid: 5'-promoter-Shine/Dalgarno-(1)-Ubiquitin-ORF-3'). This would be done because an N-terminal ubiquitin fusion can augment yield of your protein of interest ("Butt et al. 1989. "Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli." Proc. Natl. Acad. Sci. USA. 86(8):2540-2544.)
The ubiquitin can then be cleaved from your protein of interest by any of a number of ubiquitin C-terminal hydrolases (Catherine A. Gilchrist, Douglas A. Gray and Rohan T. Baker. 1997. "A Ubiquitin-specific Protease That Efficiently Cleaves the Ubiquitin-Proline Bond." Journal of Biological Chemistry. 272(51):32280-5.)
97.77.53.176 ( talk) 02:47, 7 May 2013 (UTC)
Hello fellow Wikipedians,
I have just modified 3 external links on Proteasome. Please take a moment to review my edit. If you have any questions, or need the bot to ignore the links, or the page altogether, please visit this simple FaQ for additional information. I made the following changes:
When you have finished reviewing my changes, you may follow the instructions on the template below to fix any issues with the URLs.
This message was posted before February 2018.
After February 2018, "External links modified" talk page sections are no longer generated or monitored by InternetArchiveBot. No special action is required regarding these talk page notices, other than
regular verification using the archive tool instructions below. Editors
have permission to delete these "External links modified" talk page sections if they want to de-clutter talk pages, but see the
RfC before doing mass systematic removals. This message is updated dynamically through the template {{
source check}}
(last update: 5 June 2024).
Cheers.— InternetArchiveBot ( Report bug) 16:10, 20 May 2017 (UTC)
Hello fellow Wikipedians,
I have just modified one external link on Proteasome. Please take a moment to review my edit. If you have any questions, or need the bot to ignore the links, or the page altogether, please visit this simple FaQ for additional information. I made the following changes:
When you have finished reviewing my changes, you may follow the instructions on the template below to fix any issues with the URLs.
This message was posted before February 2018.
After February 2018, "External links modified" talk page sections are no longer generated or monitored by InternetArchiveBot. No special action is required regarding these talk page notices, other than
regular verification using the archive tool instructions below. Editors
have permission to delete these "External links modified" talk page sections if they want to de-clutter talk pages, but see the
RfC before doing mass systematic removals. This message is updated dynamically through the template {{
source check}}
(last update: 5 June 2024).
Cheers.— InternetArchiveBot ( Report bug) 16:48, 24 September 2017 (UTC)
Proteasome in Sulfolobus:
Is it worth to add to the article? -- Ernsts ( talk) 16:05, 28 August 2020 (UTC)
I am reviewing this article as part of WP:URFA/2020, an initiative to review older featured articles to ensure that they still meet the featured article criteria. I have some concerns that this article does not meet the criteria anymore, which I outline below:
Is anyone willing to undertake upgrading this article? I struggle with science topics so I can only help with jargon concerns at the end of the process. Z1720 ( talk) 17:25, 17 July 2023 (UTC)