Highland Creek may refer to:

• Highland Creek (Toronto), in Scarborough, which flows into Lake Ontario
• Highland Creek, in Kenora and Rainy River districts, a tributary of the Little Turtle River
• Highland Creek, in Lambton County, which flows into Lake Huron
• Highland Creek, in Renfrew County, a tributary of the Madawaska River (Ontario)

• Highland Creek, a tributary of the Rubicon River (California)

• Highland Creek, Toronto, in Ontario, Canada
• Highland Creek (Charlotte neighborhood), a large subdivision in southern North Carolina, United States.

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