From Wikipedia, the free encyclopedia
Protein found in humans
Pre-B-cell leukemia transcription factor 1 is a
protein that in humans is encoded by the PBX1
gene .
[5] The homologous protein in Drosophila is known as extradenticle , and causes changes in embryonic development.
Function
Mice studies suggest PBX1 is involved in bone generation and skeletal patterning.
[6]
Interactions
PBX1 has been shown to
interact with:
Fruit fly homolog
The
Drosophila melangoster gene called extradenticle encodes a homeodomain protein that is 71% similar to the Pbx1 protein, and is considered homologous to PBX1.
[14] extradenticle is a
homeodomain transcription factor
[15] expressed during
embryogenesis and is related to morphological changes and development.
[14]
Reduced levels of extradenticle cause segmental transformations, without affecting the functionality or location of
homeotic genes. Complete removal of extradenticle both maternally and zygotically leads to alterations from failure of non-extradenticle protein expression.
[16]
A monoclonal antibody study of the expression of extradenticle protein in embryonic development found that it is uniformly distributed, as well as excluded from cell nuclei, until
gastrulation . During the germ band retraction stage of development, extradenticle protein begins to accumulate in the nuclei of cells in a specific pattern. Proximal areas of wing and leg
imaginal discs have extradenticle present in the nucleus, while distal areas only have it in the cytoplasm.
[17]
References
^
a
b
c
GRCh38: Ensembl release 89: ENSG00000185630 –
Ensembl , May 2017
^
a
b
c
GRCm38: Ensembl release 89: ENSMUSG00000052534 –
Ensembl , May 2017
^
"Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^
"Entrez Gene: PBX1 Pre-B-cell leukemia homeobox 1" .
^
"PBX1 PBX homeobox 1 [Homo sapiens (human)] - Gene - NCBI" . www.ncbi.nlm.nih.gov . Retrieved 2023-06-11 .
^ Berthelsen J, Zappavigna V, Ferretti E, Mavilio F, Blasi F (Mar 1998).
"The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity" . The EMBO Journal . 17 (5): 1434–45.
doi :
10.1093/emboj/17.5.1434 .
PMC
1170491 .
PMID
9482740 .
^ Piper DE, Batchelor AH, Chang CP, Cleary ML, Wolberger C (Feb 1999).
"Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation" . Cell . 96 (4): 587–97.
doi :
10.1016/S0092-8674(00)80662-5 .
PMID
10052460 .
S2CID
16122785 .
^ Chang CP, Shen WF, Rozenfeld S, Lawrence HJ, Largman C, Cleary ML (Mar 1995).
"Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins" . Genes & Development . 9 (6): 663–74.
doi :
10.1101/gad.9.6.663 .
PMID
7729685 .
^ Shen WF, Rozenfeld S, Kwong A, Köm ves LG, Lawrence HJ, Largman C (Apr 1999).
"HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells" . Molecular and Cellular Biology . 19 (4): 3051–61.
doi :
10.1128/MCB.19.4.3051 .
PMC
84099 .
PMID
10082572 .
^ Shanmugam K, Green NC, Rambaldi I, Saragovi HU, Featherstone MS (Nov 1999).
"PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins" . Molecular and Cellular Biology . 19 (11): 7577–88.
doi :
10.1128/MCB.19.11.7577 .
PMC
84774 .
PMID
10523646 .
^ Jacobs Y, Schnabel CA, Cleary ML (Jul 1999).
"Trimeric association of Hox and TALE homeodomain proteins mediates Hoxb2 hindbrain enhancer activity" . Molecular and Cellular Biology . 19 (7): 5134–42.
doi :
10.1128/MCB.19.7.5134 .
PMC
84356 .
PMID
10373562 .
^ Zubair M, Ishihara S, Oka S, Okumura K, Morohashi K (Jun 2006).
"Two-step regulation of Ad4BP/SF-1 gene transcription during fetal adrenal development: initiation by a Hox-Pbx1-Prep1 complex and maintenance via autoregulation by Ad4BP/SF-1" . Molecular and Cellular Biology . 26 (11): 4111–21.
doi :
10.1128/MCB.00222-06 .
PMC
1489093 .
PMID
16705164 .
^
a
b Rauskolb C, Peifer M, Wieschaus E (September 1993).
"extradenticle, a regulator of homeotic gene activity, is a homolog of the homeobox-containing human proto-oncogene pbx1" . Cell . 74 (6): 1101–1112.
doi :
10.1016/0092-8674(93)90731-5 .
ISSN
0092-8674 .
PMID
8104703 .
S2CID
44721382 .
^ Aspland S, White R (1997-02-01).
"Nucleocytoplasmic localisation of extradenticle protein is spatially regulated throughout development in Drosophila" . Development . 124 (3): 741–747.
doi :
10.1242/dev.124.3.741 .
ISSN
1477-9129 .
PMID
9043089 .
^ Peifer M, Wieschaus E (1990-07-01).
"Mutations in the Drosophila gene extradenticle affect the way specific homeo domain proteins regulate segmental identity" . Genes & Development . 4 (7): 1209–1223.
doi :
10.1101/gad.4.7.1209 .
ISSN
0890-9369 .
PMID
1976570 .
^
"Nucleocytoplasmic localisation of extradenticle protein is spatially regulated throughout development in Drosophila" . journals.biologists.com . Retrieved 2023-06-06 .
Further reading
Sanyal M, Tung JW, Karsunky H, Zeng H, Selleri L, Weissman IL, Herzenberg LA, Cleary ML (May 2007).
"B-cell development fails in the absence of the Pbx1 proto-oncogene" . Blood . 109 (10): 4191–9.
doi :
10.1182/blood-2006-10-054213 .
PMC
1885499 .
PMID
17244677 .
Hui H, Perfetti R (Feb 2002).
"Pancreas duodenum homeobox-1 regulates pancreas development during embryogenesis and islet cell function in adulthood" . European Journal of Endocrinology . 146 (2): 129–41.
doi :
10.1530/eje.0.1460129 .
PMID
11834421 .
Hunger SP, Galili N, Carroll AJ, Crist WM, Link MP, Cleary ML (Feb 1991).
"The t(1;19)(q23;p13) results in consistent fusion of E2A and PBX1 coding sequences in acute lymphoblastic leukemias" . Blood . 77 (4): 687–93.
doi :
10.1182/blood.V77.4.687.687 .
PMID
1671560 .
Monica K, Galili N, Nourse J, Saltman D, Cleary ML (Dec 1991).
"PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1" . Molecular and Cellular Biology . 11 (12): 6149–57.
doi :
10.1128/mcb.11.12.6149 .
PMC
361792 .
PMID
1682799 .
Nourse J, Mellentin JD, Galili N, Wilkinson J, Stanbridge E, Smith SD, Cleary ML (Feb 1990).
"Chromosomal translocation t(1;19) results in synthesis of a homeobox fusion mRNA that codes for a potential chimeric transcription factor" . Cell . 60 (4): 535–45.
doi :
10.1016/0092-8674(90)90657-Z .
PMID
1967982 .
S2CID
7975269 .
Kamps MP, Murre C, Sun XH, Baltimore D (Feb 1990). "A new homeobox gene contributes the DNA binding domain of the t(1;19) translocation protein in pre-B ALL". Cell . 60 (4): 547–55.
doi :
10.1016/0092-8674(90)90658-2 .
PMID
1967983 .
S2CID
39661371 .
Lu Q, Wright DD, Kamps MP (Jun 1994).
"Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation" . Molecular and Cellular Biology . 14 (6): 3938–48.
doi :
10.1128/mcb.14.6.3938 .
PMC
358760 .
PMID
7910944 .
Van Dijk MA, Voorhoeve PM, Murre C (Jul 1993).
"Pbx1 is converted into a transcriptional activator upon acquiring the N-terminal region of E2A in pre-B-cell acute lymphoblastoid leukemia" . Proceedings of the National Academy of Sciences of the United States of America . 90 (13): 6061–5.
Bibcode :
1993PNAS...90.6061D .
doi :
10.1073/pnas.90.13.6061 .
PMC
46867 .
PMID
8327485 .
Lu Q, Kamps MP (Apr 1996).
"Structural determinants within Pbx1 that mediate cooperative DNA binding with pentapeptide-containing Hox proteins: proposal for a model of a Pbx1-Hox-DNA complex" . Molecular and Cellular Biology . 16 (4): 1632–40.
doi :
10.1128/mcb.16.4.1632 .
PMC
231149 .
PMID
8657138 .
Berthelsen J, Zappavigna V, Mavilio F, Blasi F (Mar 1998).
"Prep1, a novel functional partner of Pbx proteins" . The EMBO Journal . 17 (5): 1423–33.
doi :
10.1093/emboj/17.5.1423 .
PMC
1170490 .
PMID
9482739 .
Berthelsen J, Zappavigna V, Ferretti E, Mavilio F, Blasi F (Mar 1998).
"The novel homeoprotein Prep1 modulates Pbx-Hox protein cooperativity" . The EMBO Journal . 17 (5): 1434–45.
doi :
10.1093/emboj/17.5.1434 .
PMC
1170491 .
PMID
9482740 .
Piper DE, Batchelor AH, Chang CP, Cleary ML, Wolberger C (Feb 1999).
"Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation" . Cell . 96 (4): 587–97.
doi :
10.1016/S0092-8674(00)80662-5 .
PMID
10052460 .
S2CID
16122785 .
Shen WF, Rozenfeld S, Kwong A, Köm ves LG, Lawrence HJ, Largman C (Apr 1999).
"HOXA9 forms triple complexes with PBX2 and MEIS1 in myeloid cells" . Molecular and Cellular Biology . 19 (4): 3051–61.
doi :
10.1128/MCB.19.4.3051 .
PMC
84099 .
PMID
10082572 .
Jacobs Y, Schnabel CA, Cleary ML (Jul 1999).
"Trimeric association of Hox and TALE homeodomain proteins mediates Hoxb2 hindbrain enhancer activity" . Molecular and Cellular Biology . 19 (7): 5134–42.
doi :
10.1128/MCB.19.7.5134 .
PMC
84356 .
PMID
10373562 .
Knoepfler PS, Bergstrom DA, Uetsuki T, Dac-Korytko I, Sun YH, Wright WE, Tapscott SJ, Kamps MP (Sep 1999).
"A conserved motif N-terminal to the DNA-binding domains of myogenic bHLH transcription factors mediates cooperative DNA binding with pbx-Meis1/Prep1" . Nucleic Acids Research . 27 (18): 3752–61.
doi :
10.1093/nar/27.18.3752 .
PMC
148632 .
PMID
10471746 .
McWhirter JR, Neuteboom ST, Wancewicz EV, Monia BP, Downing JR, Murre C (Sep 1999).
"Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia" . Proceedings of the National Academy of Sciences of the United States of America . 96 (20): 11464–9.
Bibcode :
1999PNAS...9611464M .
doi :
10.1073/pnas.96.20.11464 .
PMC
18056 .
PMID
10500199 .
Shanmugam K, Green NC, Rambaldi I, Saragovi HU, Featherstone MS (Nov 1999).
"PBX and MEIS as non-DNA-binding partners in trimeric complexes with HOX proteins" . Molecular and Cellular Biology . 19 (11): 7577–88.
doi :
10.1128/MCB.19.11.7577 .
PMC
84774 .
PMID
10523646 .
Mikkola I, Bruun JA, Holm T, Johansen T (Feb 2001).
"Superactivation of Pax6-mediated transactivation from paired domain-binding sites by dna-independent recruitment of different homeodomain proteins" . The Journal of Biological Chemistry . 276 (6): 4109–18.
doi :
10.1074/jbc.M008882200 .
PMID
11069920 .
Thameem F, Wolford JK, Bogardus C, Prochazka M (Mar 2001).
"Analysis of PBX1 as a candidate gene for type 2 diabetes mellitus in Pima Indians" . Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression . 1518 (1–2): 215–20.
doi :
10.1016/S0167-4781(01)00189-0 .
PMID
11267683 .
Liu Y, MacDonald RJ, Swift GH (May 2001).
"DNA binding and transcriptional activation by a PDX1.PBX1b.MEIS2b trimer and cooperation with a pancreas-specific basic helix-loop-helix complex" . The Journal of Biological Chemistry . 276 (21): 17985–93.
doi :
10.1074/jbc.M100678200 .
PMID
11279116 .
Mal A, Sturniolo M, Schiltz RL, Ghosh MK, Harter ML (Apr 2001).
"A role for histone deacetylase HDAC1 in modulating the transcriptional activity of MyoD: inhibition of the myogenic program" . The EMBO Journal . 20 (7): 1739–53.
doi :
10.1093/emboj/20.7.1739 .
PMC
145490 .
PMID
11285237 .
External links
PDB gallery
1b72 : PBX1, HOMEOBOX PROTEIN HOX-B1/DNA TERNARY COMPLEX
1b8i : STRUCTURE OF THE HOMEOTIC UBX/EXD/DNA TERNARY COMPLEX
1du6 : SOLUTION STRUCTURE OF THE TRUNCATED PBX HOMEODOMAIN
1lfu : NMR solution structure of the extended PBX homeodomain bound to DNA
1puf : Crystal structure of HoxA9 and Pbx1 homeodomains bound to DNA
(1) Basic domains
(1.1) Basic
leucine zipper (
bZIP )(1.2) Basic helix-loop-helix (
bHLH )
Group A Group B Group C bHLH-
PAS Group D Group E Group F bHLH-COE
(1.3)
bHLH-ZIP (1.4) NF-1 (1.5) RF-X (1.6) Basic helix-span-helix (bHSH)
(2)
Zinc finger DNA-binding domains
(2.1)
Nuclear receptor (Cys4 )
subfamily 1 subfamily 2 subfamily 3 subfamily 4 subfamily 5 subfamily 6 subfamily 0
(2.2) Other Cys4 (2.3) Cys2 His2 (2.4) Cys6 (2.5) Alternating composition (2.6) WRKY
(4) β-Scaffold factors with minor groove contacts
(0) Other transcription factors