This protein is a part of a SCF complex consisting of CUL1,
RBX1,
SKP1 and
SKP2. It also interacts with
RNF7. Part of a complex with
TIP120A/
CAND1 and
RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex. Interacts with
COPS2.
The protein is
neddylated, which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the
COP9 signalosome (CSN) complex.
Michel JJ, Xiong Y (1998). "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A.". Cell Growth Differ. 9 (6): 435–49.
PMID9663463.
Marti A, Wirbelauer C, Scheffner M, Krek W (1999). "Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation". Nat. Cell Biol. 1 (1): 14–9.
doi:
10.1038/8984.
PMID10559858.
S2CID8884226.
Hori T, Osaka F, Chiba T, et al. (2000). "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene. 18 (48): 6829–34.
doi:
10.1038/sj.onc.1203093.
PMID10597293.
S2CID22861534.
Morimoto M, Nishida T, Honda R, Yasuda H (2000). "Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the activity of SCF(skp2) toward p27(kip1)". Biochem. Biophys. Res. Commun. 270 (3): 1093–6.
doi:
10.1006/bbrc.2000.2576.
PMID10772955.
Swaroop M, Wang Y, Miller P, et al. (2000). "Yeast homolog of human SAG/ROC2/Rbx2/Hrt2 is essential for cell growth, but not for germination: chip profiling implicates its role in cell cycle regulation". Oncogene. 19 (24): 2855–66.
doi:
10.1038/sj.onc.1203635.
PMID10851089.
S2CID2100588.