4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470
Amido black 10B is an
amino acidstainingazo dye used in biochemical research to stain for total
protein on transferred membrane blots, such as the
western blot.[2] It is also used in criminal investigations to detect
blood present with latent
fingerprints - it stains the proteins in blood a blue-black color.[3] Amido Black can be either
methanol or water based as it readily dissolves in both. With
picric acid, in a
van Gieson procedure, it can be used to stain
collagen and
reticulin.
^Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer.
ISBN978-1-4939-2694-7
^Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11.
doi:
10.1016/j.forsciint.2011.04.006